Overview In this role you will lead LC-MS-based characterization and quantitation for ADCs, antibodies, and small molecules to support oncology discovery. You collaborate with cross-functional teams to develop and validate analytical methods, troubleshoot challenges, and push method performance. You will work hands-on with advanced instrumentation to enable early development programs and present findings to project teams. This is a hands-on, impact-focused position at the interface of analytical science and drug discovery.
Responsibilities- Perform analytical characterization and QC of ADCs, antibodies, and small molecules using LC-MS and HPLC (intact mass, de-glycosylation, reducing LC-MS, DAR, SEC, HIC, RP-HPLC, peptide mapping)
- Prepare and analyze samples from purified proteins and biological matrices (plasma, serum, tissues)
- Identify and characterize ADC metabolites from in vitro and in vivo studies using affinity capture, forced release, MS/MS, and SIM-MS
- Develop, optimize, validate, and troubleshoot analytical methods to improve performance
- Operate, maintain, and troubleshoot analytical instrumentation (Thermo Vanquish UHPLC-Orbitrap MS, Waters Acquity UPLC-SQD MS, Agilent HPLC, UV-Vis)
- Evaluate and recommend new analytical technologies to expand capabilities
- Document work in ELNs, maintain analytical databases, and prepare reports/project summaries
- Present results to multidisciplinary project teams
- Support lab operations and optimize analytical workflows and equipment utilization
Key requirements- Ph.D. in Analytical chemistry or related field with minimum 5 years' experience (MS 8 years, BS 11 years)
- Industry experience in biopharmaceutical/pharmaceutical analytical chemistry
- Hands-on LC-MS, UV-Vis, and HPLC characterization of proteins and bioconjugates
- Protein sample prep including deglycosylation, reduction, and enzymatic digestions of ADCs
- Working knowledge of SEC, HIC, and reversed-phase HPLC
- Experience operating, maintaining, and troubleshooting LC-MS and HPLC instruments
- Strong analytical and problem-solving skills with attention to detail
- Ability to manage multiple priorities and work independently in a fast-paced environment
- Excellent written and verbal communication and ability to present data to cross-functional teams
- Demonstrated collaboration with chemists, protein engineers, and biologists
- Scientific curiosity and interest in emerging analytical technologies
- Publication record in peer-reviewed journals is desirable
- attention to detail
- excellent communication
- team collaboration
- LC-MS
- HPLC
- de-glycosylation
